Introduction: The satisfaction levels of nurses directly impact patient outcomes, highlighting the importance of assessing nurses’ job satisfaction to identify areas for improvement and enhance patient care. Therefore, this study aimed to evaluate the job satisfaction levels among nurses working in a tertiary care setting. Methods: Job satisfaction was assessed using the Minnesota Satisfaction Questionnaire (MSQ-20), a tool commonly used in healthcare settings for nurses, comprising 20 items related to intrinsic and extrinsic factors. A total of 60 samples were collected using convenience sampling techniques. Results: The findings indicated a significant difference in job satisfaction between single and married nurses (p = 0.012), with married nurses showing higher satisfaction levels. Moreover, nurses aged 50–60 exhibited higher job satisfaction compared to those aged 20–30 (p = 0.049). Additionally, nurses with 15–20 years of experience at the same hospital reported higher satisfaction levels compared to those with 2–3 years of tenure (p = 0.037). Designation was strongly associated with extrinsic factors of job satisfaction (p = 0.008). Conclusion: The study found that married nurses, those aged 50–60, and nurses with 15–20 years of experience at the same hospital reported significantly higher job satisfaction. Designation was also strongly linked to extrinsic factors influencing job satisfaction. The study's findings offer valuable insights for hospital administrators, nurse leaders, and policymakers to enhance nurse job satisfaction and improve overall healthcare outcomes.