Citation Information :
Kumawat S, Mamoria VP, Sharma R. Prevalence and Identification of Nonfermentative Gram-negative Bacilli among Patients at Tertiary Care Hospital, Jaipur. J Mahatma Gandhi Univ Med Sci Tech 2024; 9 (1--3):1-4.
Aims and objectives: Nonfermentative gram-negative bacilli (NFGNB) cause wide spectrum of infections, including nosocomial pneumonia, secondary meningitis, surgical wound infections, skin and soft tissue infection, urinary tract infection, septicemia, and transmission via the hand of hospital personnel. Most of the nonfermenters cause nosocomial bloodstream infections, particularly in debilitated and immunocompromised hosts. The study aimed to prevalence and identification of NFGNB isolates in the clinical samples among patients at Mahatma Gandhi Medical College and Hospital.
Introduction: In recent decades, infections most commonly caused by Pseudomonas aeruginosa and Acinetobacter baumannii have also occurred outside the intensive care unit (ICU) or in trauma patients after natural disasters and they have even affected patients after comorbidities in the community.
Materials and methods: All NFGNB isolates from different clinical samples received in clinical microbiology laboratory from outpatients & inpatients Mahatma Gandhi Medical College and Hospital, Jaipur, Rajasthan, were included in the study. Routine microscopy of samples was done. Gram staining was done for all samples except urine. All clinical samples were inoculated on blood agar and MacConkey agar was incubated at 37° for 18–24 hours. Colony characteristics were observed. All the organisms that produced pale or colorless colonies on MacConkey agar and also showed GNB on Gram staining of the colonies were considered to be NFGNB and further identified by VITEK 2 compact system.
Results: Among 10,707 samples, 879 NFGNB isolates were obtained, so the prevalence of NFGNB in the study was 8.20%. The result was analyzed as follows: out of 879 NFGNB isolates were obtained, of which 415 (47.21%) were Pseudomonas aeruginosa, followed by Acinetobacter baumannii 378 (43.00%), Burkholderia cepacia 29 (3.30%), Burkholderia pseudomallei 17 (1.93%), Stenotrophomonas maltophilia 16 (1.82%), Acinetobacter lwoffii 7 (0.80%), Pseudomonas putida 6 (0.68%), Acinetobacter coffee 2 (0.23%), Acinetobacter fwinii 2 (0.23%), Pseudomonas fluorescens 2 (0.23%), Pseudomonas stutzeri 2 (0.23%), Acinetobacter junii 1 (0.11%), Acinetobacter haemolyticus 1 (0.11%), and Pseudomonas luteola 1 (0.11%).
Conclusion: Based on this study, it could be concluded hardships will be experienced in all NFGNB treatments unless the necessary precautions are taken. In order to prevent the spreading of infection control measures should be taken, and clinicians and hospital hygienic rules should be observed.
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