Introduction: We are all biologically unique and become more diverse as we age, enhancing the spice of life with increasing variety. In many ways, the teeth are unique organs of the body; they are the most durable part of the skeleton. The gradual changes taking place in the dental tissue after the teeth are fully formed are of clinical importance and need to be recognized by the dental surgeon as being a normal finding and not a part of the disease process. Aims and Objectives: The aim of this study is to estimate relationship between size of the pulp and the dentin with age and to know if there is a significant difference in size of the pulp and the dentin among various age groups. The clinical significance of the study will be to use the average tooth length and width obtained by this study as the standard guideline for age estimation and to utilize the results of the present study in forensic science and anthropology and as an aging biomarker. Results: From the study, it was concluded that in both males and females there is decrease in root canal length and width with increasing age, which is attributed to secondary dentin formation. In the age group of 11–60, the length and width of pulp canals of mandibular central and lateral incisors decreased with the advancing age and this difference is statistically significant with the p value of less than 0.000. Conclusion: In the future computer-assisted measurements, i.e., latest digital radiographic techniques are suggested to measure the above parameters; this may could throw light on forensic applications, medicolegal issues, and legal issues regarding age estimation by measuring root canal length and width.