Introduction: Dengue is a major health threat with epidemic potential and is prevalent in tropical and subtropical regions of the world. This study was aimed at analyzing the circulating dengue serotypes in our region and their correlation with the severity of disease presenting to our hospital. Materials and methods: A hospital-based retrospective study was done on 100 patients with dengue infection who fulfilled the inclusion and exclusion criteria, admitted under the Department of General Medicine of Mahatma Gandhi Medical College and Hospital, Jaipur. Dengue serotype was confirmed by RT-PCR. Results: A total of 100 patients were included in the data analysis. Adult age group (82%) and male gender (54%) were the most commonly affected group. The most common serotypes in circulation were DENV-2 and DENV-3 (60% cases). Severe dengue was maximum in patients with DENV-2 (20.6%). Concurrent infection with two serotypes was present in four cases. The mean duration of hospital stay was maximum in DENV-4 (5.33 ± 2.09 days). The maximum percentage of DENV-1 cases required platelet transfusion. Conclusion: At our hospital, adult age group and male gender are most commonly affected, with DENV-2 being the most common serotype and having maximum severity. Initial serotyping of dengue patients can help monitor the epidemiological and clinical trends of the different serotypes of dengue infection. Co-infection with two serotypes can also occur.