Journal of Mahatma Gandhi University of Medical Sciences and Technology

Register      Login

VOLUME 5 , ISSUE 2 ( May-August, 2020 ) > List of Articles

Original Article

Evaluation of Phytochemicals Found in the Leaves of Jasminum sambac L. for Antimicrobial Activity

Kamakshi Tomar, Shilpi Rijhwani

Keywords : Antimicrobial activity, Jasminum sambac, Phytochemicals

Citation Information : Tomar K, Rijhwani S. Evaluation of Phytochemicals Found in the Leaves of Jasminum sambac L. for Antimicrobial Activity. J Mahatma Gandhi Univ Med Sci Tech 2020; 5 (2):57-60.

DOI: 10.5005/jp-journals-10057-0128

License: CC BY-NC 4.0

Published Online: 12-04-2021

Copyright Statement:  Copyright © 2020; Jaypee Brothers Medical Publishers (P) Ltd.


Abstract

Jasminum sambac Linn. (Family—Oleaceae) was tested for antibacterial activity against three important pathogenic bacteria, i.e., Staphylococcus aureus, Escherichia coli, and Pseudomonas aeruginosa. Powdered leaf material was extracted with different solvents from non-polar to polar viz., n-hexane, chloroform, ethyl acetate, methanol, and water using Soxhlet apparatus. All the solvent extracts were evaporated to dryness using a hot dry air oven. The dry residue was dissolved in respective mother solvents (1:10 w/v) and tested for the presence or absence of various phytochemicals. An antimicrobial test was also followed to check the antimicrobial activity. Among five solvents tested, methanol, n-hexane, and ethyl acetate extracts showed significant antibacterial activity and chloroform extract showed less activity when compared with control (respective mother solvent). Water extract did not show any activity against all three microorganisms.


PDF Share
  1. Edris AE. Pharmaceutical and therapeutic potentials of essential oils and their individual volatile constituents: a review. Phytother Res (Press) 2007;21(4):308–323. DOI: 10.1002/ptr.2072.
  2. Cowan MM. Plant products as antimicrobial agents. Clin Microbiol Rev 1999;12(4):564–582. DOI: 10.1128/CMR.12.4.564.
  3. Ahmad I, Mehmood J, Mohammad F. Screening of some Indian medicinal plants for their antimicrobial properties. J Ethnopharmacol 1998;62(2):183–193. DOI: 10.1016/S0378-8741(98)00055-5.
  4. Arora DS, Kaur J. Antimicrobial activity of spices. Int J Antimicrob Agents 1999;12(3):257–262. DOI: 10.1016/S0924-8579(99)00074-6.
  5. Arora DS, Kaur GJ, Kaur H. Antibacterial activity of tea and coffee: their extracts and preparations. Int J Food Prop 2009;12(2):286–294. DOI: 10.1080/10942910701675928.
  6. Rojas JJ, Ochoa VJ, Ocampo SA, et al. Screening for antimicrobial activity of ten medicinal plants used in colombian folkloric medicine: a possible alternative in the treatment of non-nosocomial infections. BMC Complement Altern Med 2006;6(1):2. DOI: 10.1186/1472-6882-6-2.
  7. Iwu MW, Duncan AR, Okunjii CO. New antimicrobials of plant origin. In: Perspect N Crop N uses Alexandria JJ, ed., Virginia: ASHS Press; 1999. pp. 457–462.
  8. McFarland J. Nephelometer: an instrument for estimating the number of bacteria in suspensions used for calculating the opsonic index and for vaccines. J Am Med Assoc 1907;14(14):1176–1178. DOI: 10.1001/jama.1907.25320140022001f.
  9. Bauer AW, Kirby WM, Sherris JC, et al. Antibiotic susceptibility testing by a standard single disk method. Am J Clin Path 1966;45:493–496.
  10. Harborne JB. Phytochemical Methods. 3rd ed., London: Chapman and Hall; 1998. pp. 1–302. ISBN: 0-412-57260-5.
  11. Vaghasiya Y, Dave R, Chanda A. Phytochemical analysis of some medicinal plants from western region of India. Res J Med Plant 2011;5(5):567–576. DOI: 10.3923/rjmp.2011.567.576.
  12. Evans WC. Trease and Evans Pharmacognosy. 14th ed., WB Saunders Company Ltd.; 2000. pp. 224–293.
  13. Didry N, Dubreuil L, Trotin F, et al. Antimicrobial activity of the aerial parts of drosera peltata smith on oral bacteria. J Ethnopharmacol 1998;60(1):91–96. DOI: 10.1016/S0378-8741(97)00129-3.
  14. Srinivasan D, Nathan S, Suresh T, et al. Antimicrobial activity of certain Indian medicinal plants used in folkloric medicine. J Ethnopharmacol 2001;74(3):217–220. DOI: 10.1016/S0378-8741(00)00345-7.
  15. Heisig P. Planta Med 2001;67:4–12.
  16. Aiyelaagbe OO. Antibacterial activity of jatrophamultifidaroots. Fitoterapia 2000;72(5):544–546. DOI: 10.1016/S0367-326X(00)00291-4.
  17. Prashanth D, Asha MK, Amit A. Antibacterial activity of punicagranatum. Fitoterapia 2001;72(2):171–173. DOI: 10.1016/S0367-326X(00)00270-7.
  18. Mounissamy V, Davimani S, Gunasegaran R. Antibacterial activity of gossyptein isolated from hibiscus saddriffa. Antiseptic 2002;99:81–82.
  19. Woldemichael GM, Wächter G, Singh MP, et al. Antibacterial diterpenes from calceolaria pinifolia. J Nat Prod 2003;66(2):242–246. DOI: 10.1021/np020380x.
  20. Satish S, Raveesha KA, Janardhana GR, et al. Lett Appl Microbiol 1999;28(2):145–147. DOI: 10.1046/j.1365-2672.1999.00479.x.
  21. Bisignano G, Sanogo R, Marino A, et al. Antimicrobial activity of Mitracarpusscaber extract and isolated constituents. Lett Appl Microbiol 2000;30(2):105–108. DOI: 10.1046/j.1472-765x.2000.00692.x.
  22. Mishra A. Studies on biochemical and physiological aspects in relation to phyto-medicinal qualities and efficacy of the active ingredients during the handling, cultivation and harvesting of medicinal plants. J Med Plants Res 2009;3:1140–1146.
  23. Jana S, Shekhawat GS. Phytochemical analysis and antibacterial screening of in vivo and in vitro extracts of Indian medicinal herb: anethumgraveolens. Res J Med Plant 2010;4(4):206–212. DOI: 10.3923/rjmp.2010.206.212.
  24. Ivanova D, Gerova D, Chervenkov T, et al. Polyphenols and antioxidant capacity of Bulgarian medicinal plants. J Ethnopharmacol 2005;96(1–2):145–150. DOI: 10.1016/j.jep.2004.08.033.
  25. Shrikumar S, Ravi TK. Approaches towards development and promotion of herbal drugs. Phcog Rev 2007;1:180–184.
  26. Mandal V, Mohan Y, Hemalatha S. Microwave assisted extraction - an innovative and promising extraction tool for medicinal plant research. Pharmacog Rev 2007;1:7–18.
  27. Jeyachandran R, Mahesh A. Enumeration of antidiatic herbal flora of Tamilnadu. Res J Med Plant 2007;1(4):144–148. DOI: 10.3923/rjmp.2007.144.148.
PDF Share
PDF Share

© Jaypee Brothers Medical Publishers (P) LTD.