Biofilm Production in Clinical Isolates and Their Antimicrobial Susceptibility Pattern in the Intensive Care Unit of a Tertiary Care Hospital in Rajasthan
Neha Gupta, Malvika Sharma, Shanoo Sharma
Antibiotic susceptibility, Biofilms, Device-associated infections, Quorum sensing, Resistance
Citation Information :
Gupta N, Sharma M, Sharma S. Biofilm Production in Clinical Isolates and Their Antimicrobial Susceptibility Pattern in the Intensive Care Unit of a Tertiary Care Hospital in Rajasthan. J Mahatma Gandhi Univ Med Sci Tech 2017; 2 (2):55-60.
Aims and objectives
This study was carried out to know about the production of biofilm by the microorganisms in various clinical isolates and to compare the antimicrobial sensitivity pattern of biofilm- and nonbiofilm-producing organisms.
Materials and methods
One hundred and fifty samples collected from intensive care units for a period of 1 year were taken for the study. Samples included blood, urine, sputum, endotracheal tips, suction tips, pus/swabs, stents/valves, body fluids, etc. Samples were processed and identification of microorganisms and antibiotic sensitivity was tested by methods according to Clinical and Laboratory Standards Institute guidelines.
Biofilm production identification was done by tissue culture plate (TCP) method, tube method (TM), and Congo red agar (CRA) plate method.
Out of 150 samples, 108 (72%) samples showed growth of Gram-negative bacilli, 16 (11%) samples showed growth of Gram-positive cocci, and Candida species were seen in remaining 26 (17%) samples. Among the total organisms isolated, 124 organisms (82.66%) showed production of biofilm, while 26 organisms (17.33%) did not produce biofilm. Antibiotic resistance was seen more in biofilm-producing organisms as compared with nonbiofilm-producing organisms.
Most of the biofilm-related infections are characterized particularly by high resistance to antibiotics and persistent infections, in turn leading to a very high morbidity and mortality. Therefore, detection of biofilm production is of high relevance to the clinician for appropriate approach to the treatment.
How to cite this article
Sharma M, Gupta S, Gupta N, Sharma S. Biofilm Production in Clinical Isolates and Their Antimicrobial Susceptibility Pattern in the Intensive Care Unit of a Tertiary Care Hospital in Rajasthan. J Mahatma Gandhi Univ Med Sci Tech 2017;2(2):55-60.
Rao GP. Spinal intramedullary tuberculous lesion: medical management. Report of four cases. J Neurosurg 2000;93(Suppl 1):137-141.
Jena A, Banerji AK, Tripathi RP, Gulati PK, Jain RK, Khushu S, Sapra ML. Demonstration of intramedullary tuberculomas by magnetic resonance imaging: a report of two cases. Br J Radiol 1991;64(762):555-558.
Citow JS, Ammirati M. Intramedullary tuberculoma of the spinal cord: case report. Neurosurgery 1994 Aug;35(2):327-330.
Medhi S, Goswami B, Das AK, Singh TB, Husain SA, Sehgal A, Kar P. New insights into hepatitis C virus infection in the tribal-dominant part of Northeast India. Arch Virol 2012 Nov;157(11):2083-2093.
Raghuraman S, Abraham P, Sridharan G, Ramakrishna BS. Hepatitis C virus genotype 6 infection in India. Indian. J Gastroenterol 2005 Mar-Apr;24(2):72-75.
Raghuraman S, Abraham P, Sridharan G, Daniel HD, Ramakrishna BS, Shaji RV. HCV genotype 4 — an emerging threat as a cause of chronic liver disease in Indian (south) patients. J Clin Virol 2004 Dec;31(4):253-258.
Umer M, Iqbal M. Hepatitis C virus prevalence and genotype distribution in Pakistan: comprehensive review of recent data. World J Gastroenterol 2016 Jan;22(4):1684-1700.
Esmaeilzadeh A, Erfanmanesh M, Ghasemi S, Mohammadi F. Serological assay and genotyping of hepatitis C virus in infected patients in Zanjan province. Hepat Mon 2014 Sep;14(9):e17323.
Mittal G, Gupta P, Gupta R, Ahuja V, Mittal M, Dhar M. Seroprevalence and risk factors of hepatitis B and hepatitis C virus infections in Uttarakhand, India. J Clin Exp Hepatol 2013 Dec;3(4):296-300.
Sarma MP, Asim M, Medhi S, Bharathi T, Diwan R, Kar P. Viral genotypes and associated risk factors of hepatocellular carcinoma in India. Cancer Bio Med 2012 Sep;9(3):172-181.
Ullah, Mi Hasan, F Najmudin, Alam, MM SSZZ, Rana, MS Nawaz, M Rehman S and Shah A A. Seroprevalence of Hepatitis C Virus Infection in Kohat Division, Khyber Pakhtoonkhwa, Pakistan. Pakistan J Zool 2016;.48(6):1721-1725.
Ramarokoto CE, Rakotomanana F, Ratsitorahina M, Raharimanga V, Razafindratsimandresy R, Randremanana R, Rakoto-Andrianarivelo M, Rousset D, Andrianaja V, Richard V, et al. Seroprevalence of hepatitis C and associated risk factors in urban areas of Antananarivo, Madagascar. BMC Infect Dis 2008 Feb;8:25.
Singh P, Kaur R, Kaur A. Frequency distribution of Hepatitis C virus in different geographical regions of Punjab: retrospective study from a tertiary care centre in North India. J Nat Sci Biol Med 2014 Jan;5(1):56-58.
Sandhu R, Dahiya S. Prevalence of anti-hepatitis C virus antibodies among inpatients and outdoor attendees of a tertiary care institute. Br Biomed Bull 2015 Jan;3(1):8-14.
Da Ros CT, Schmitt CdaS. Global epidemiology of sexually transmitted diseases. Asian J Androl 2008 Jan;10(1): 110-114.
Te HS, Jensen DM. Epidemiology of hepatitis B and C viruses: a global overview. Clin Liver Dis 2010 Feb;14(1):1-21.
Khan IA, Pilli S A S, Rampal R, Chauhan SK, Tiwari V, Mouli VP, Kedia S, Nayak B, Das P, Makharia GK, et al. Prevalence and Association of Mycobacterium avium subspecies paratuberculosis with disease course in patients with ulcero-constrictive ileocolonic disease. PLoS One 2016 Mar;11(3):e0152063.
Ramadass B, Chittaranjan S, Subramanian V, Ramakrishna BS. Fecal polymerase chain reaction for Mycobacterium tuberculosis IS6110 to distinguish Crohn's disease from intestinal tuberculosis. Indian J Gastroenterol 2010 Jul;29(4):152-156.
Jin XJ, Kim JM, Kim HK, Kim L, Choi SJ, Park IS, Han JY, Chu YC, Song JY, Kwon KS, et al. Histopathology and TB-PCR kit analysis in differentiating the diagnosis of intestinal tuberculosis and Crohn's disease. World J Gastroenterol 2010 May;16(20):2496-2503.
Jin T, Fei B, Zhang Y, He X. The diagnostic value of polymerase chain reaction for Mycobacterium tuberculosis to distinguish intestinal tuberculosis from Crohn's disease: a meta-analysis. Saudi J Gastroenterol 2017 Jan-Feb;23(1):3-10.
Sechi LA, Mura M, Tanda F, Lissia A, Solinas A, Fadda G, Zanetti S. Identification of Mycobacterium avium subsp. paratuberculosis in biopsy specimens from patients with Crohn's disease identified by in situ hybridization. J Clin Microbiol 2001 Dec;39(12):4514-4517.
Sechi LA, Mura M, Tanda E, Lissia A, Fadda G, Zanetti S. Mycobacterium avium sub. paratuberculosis in tissue samples of Crohn's disease patients. New Microbiol 2004 Jan;27(1):75-77.
Sechi LA, Scanu AM, Molicotti P, Cannas S, Mura M, Dettori G, Fadda G, Zanetti S. Detection and Isolation of Mycobacterium avium subspecies paratuberculosis from intestinal mucosal biopsies of patients with and without Crohn's disease in Sardinia. Am J Gastroenterol 2005 Jul;100(7):1529-1536.
Pulimood AB, Peter S, Rook GW, Donoghue HD. In situ PCR for Mycobacterium tuberculosis in endoscopic mucosal biopsy specimens of intestinal tuberculosis and Crohn disease. Am J Clin Pathol 2008 Jun;129(6):846-851.
Aimaiti R, Qin L, Cao T, Yang H, Wang J, Lu J, Huang X, Hu Z. Identification and application of ssDNA aptamers against H37Rv in the detection of Mycobacterium tuberculosis. Appl Microbiol Biotechnol 2015 Nov;99(21):9073-9083.
Mozioglu E, Gokmen O, Tamerler C, Kocagoz ZT, Akgoz M. Selection of nucleic acid aptamers specific for Mycobacterium tuberculosis. Appl Biochem Biotechnol 2016 Feb;178(4):849-864.
Tang XL, Wu SM, Xie Y, Song N, Guan Q, Yuan C, Zhou X, Zhang XL. Generation and application of ssDNA aptamers against glycolipid antigen ManLAM of Mycobacterium tuberculosis for TB diagnosis. J Infect 2016 May;72(5):573-586.
Pan Q, Wang Q, Sun X, Xia X, Wu S, Luo F, Zhang XL. Aptamer against mannose-capped lipoarabinomannan inhibits virulent Mycobacterium tuberculosis infection in mice and rhesus monkeys. Mol Ther 2014 May;22(5):940-951.
Perkins AC, Missailidis S. Radiolabelled aptamers for tumour imaging and therapy. Q J Nucl Med Mol Imaging 2007 Dec;51(4):292-296.
Calzada V, Moreno M, Newton J, González J, Fernández M, Gambini JP, Ibarra M, Chabalgoity A, Deutscher S, Quinn T, et al. Development of new PTK7-targeting aptamer-fluorescent and radiolabelled probes for evaluation as molecular imaging agents: lymphoma and melanoma in vivo proof of concept. Bioorg Med Chem 2016 Dec;25(3):1163-1171.
Lele, RD. Imaging tuberculosis with radiolabelled aptamers. In: Kulkarni SV, editor. Pursuit of excellence—an autobiography. Mumbai: Granthali Woollen Mill Public School; 2017. pp. 154-156.
Fogel R, Limson J. Developing biosensors in developing countries: South Africa as a case study. Biosensors (Basel) 2016 Feb;6(1):E5.
Chen F, Zhou J, Luo F, Mohammed AB, Zhang XL. Aptamer from whole-bacterium SELEX as new therapeutic reagent against virulent Mycobacterium tuberculosis. Biochem Biophys Res Commun 2007 Jun;357(3):743-748.
Baig IA, Moon JY, Lee SC, Ryoo SW, Yoon MY. Development of ssDNA aptamers as potent inhibitors of Mycobacterium tuberculosis acetohydroxy acid synthase. Biochim Biophys Acta 2015 Oct;1854(10 Pt A):1338-1350.
Sohal JS, Sheoran N, Narayanasamy K, Brahmachari V, Singh S, Subodh S. Genomic analysis of local isolate of Mycobacterium avium subspecies paratuberculosis. Vet Microbiol 2009 Mar;134(3-4):375-382.